American Chemical Society
An Introduction to Computer Searching of
Chemical Abstracts
STN International Version
Biochemistry Sample Search
Search Topic:
Find references on the use of antibodies to study acetylcholine receptors.
Strategy:
- Find Registry Number for Acetylcholine
- Key terms:
- Acetylcholine or its Registry Number
- receptor# (The # symbol acts as one or zero characters; this retrieves receptor, receptors)
- antibod? (retrieves antibody, anitbodies)
- Link terms with (s) operator for greater precision
- Display scan a few references to check for additional relevant terms.
- Add additional synonyms for acetycholine-related receptors: cholinergic, nicotinic, muscarinic
Teaching Points:
Registry File:
- use CN Field to find registry number (RN)
- verify that name appears
- SEARCH to obtain RN
CA File:
- limited truncation
- (S) operator for more precise searching
- D SCAN to identify additional search terms
- adding terms to search a strategy
Search Strategy Table
| Search Steps | Set Number | Number of Documents |
|---|---|---|
| File reg | ||
| e acetylcholine/cn | ||
| s E3 | L1 | 1 |
| d L1 ide 1 | ||
| file ca | ||
| s L1 or acetylcholine | L2 | 41310 |
| s receptor# | L3 | 319187 |
| s antibod? | L4 | 206193 |
| s L2 (s) L3 (s) L4 | L5 | 757 |
| d L5 scan | ||
| s muscarinic or nicotinic or cholinergic | L6 | 49216 |
| s (L2 or L6) (s) L3 (s) L4 | L7 | 938 |
| s L7 not L5 | L8 | 181 |
| d L8 ibib 1 | ||
| log y |
Sample Search
Search in Registry File for Registry Numbers for acetylcholine
=> file reg FILE ‘REGISTRY’ ENTERED AT 17:36:18 ON 04 JUN 96 USE IS SUBJECT TO THE TERMS OF YOUR CUSTOMER AGREEMENT COPYRIGHT (C) 1996 American Chemical Society (ACS) STRUCTURE FILE UPDATES: 31 MAY 96 HIGHEST RN 176894-09-0 DICTIONARY FILE UPDATES: 3 JUN 96 HIGHEST RN 176894-09-0 TSCA INFORMATION NOW CURRENT THROUGH DECEMBER 1995
Expand on acetylcholine in the Chemical Name (cn) field to verify the form of the name of the Registry file
=> e acetylcholine/cn E1 1 ACETYLCHLOROSULFISOMIDINE/CN E2 1 ACETYLCHOLIN-RETARD/CN E3 1 --> ACETYLCHOLINE/CN E4 1 ACETYLCHOLINE + ESERINE/CN E5 1 ACETYLCHOLINE .BETA.-RESORCYLATE/CN E6 1 ACETYLCHOLINE 2-NITROBENZOATE/CN E7 1 ACETYLCHOLINE 3-CHLOROBENZOATE/CN E8 1 ACETYLCHOLINE 3-PHENYLPROPIONATE/CN E9 1 ACETYLCHOLINE 4-NITROBENZOATE/CN E10 1 ACETYLCHOLINE ACETYLHYDROLASE/CN E11 1 ACETYLCHOLINE BENZENESULFONATE/CN E12 1 ACETYLCHOLINE BENZOATE/CN
Search the E# from the expand list to save retyping acetylcholine
=> s e3 L1 1 ACETYLCHOLINE/CN
Display answer 1 of set one in ide format
=> d l1 ide 1
L1 ANSWER 1 OF 1 REGISTRY COPYRIGHT 1996 ACS
RN 51-84-3 REGISTRY
CN Ethanaminium, 2-(acetyloxy)-N,N,N-trimethyl- (9CI)
(CA INDEX NAME)
OTHER CA INDEX NAMES:
CN Choline acetate (ester) (8CI)
CN Choline, acetate (7CI)
OTHER NAMES:
CN Acetyl choline ion
CN Acetylcholine
CN Acetylcholine cation
CN Acetylcholinium
CN ACh
CN Choline, acetyl-
FS 3D CONCORD
MF C7 H16 N O2
CI COM
LC STN Files: AIDSLINE, ANABSTR, BEILSTEIN*,
BIOBUSINESS, BIOSIS, CA, CABA, CANCERLIT, CAOLD,
CAPLUS, CAPREVIEWS, CASREACT, CEN, CHEMLIST, CIN,
CJACS, CSNB, DDFU, DRUGU, EMBASE, GMELIN*, IFICDB,
IFIPAT, IFIUDB, IPA, MEDLINE, NAPRALERT, PNI, PROMT,
RTECS*, SPECINFO, TOXLINE, TOXLIT, USAN, USPATFULL,
VETU (*File contains numerically searchable property data)
Other Sources: EINECS**
(**Enter CHEMLIST File for up-to-date regulatory information)
Me3+N CH2 CH2 OAc
......... ....... .......
32 REFERENCES IN FILE CAPREVIEWS
21223 REFERENCES IN FILE CA (1967 TO DATE)
85 REFERENCES TO NON-SPECIFIC DERIVATIVES IN FILE CA
21260 REFERENCES IN FILE CAPLUS (1967 TO DATE)
1 REFERENCES IN FILE CAOLD (PRIOR TO 1967)
Transfer to the CA file to search for relevant articles
=> file ca FILE ‘CA’ ENTERED AT 19:06:11 ON 24 JUN 96 USE IS SUBJECT TO THE TERMS OF YOUR CUSTOMER AGREEMENT COPYRIGHT (C) 1996 AMERICAN CHEMICAL SOCIETY (ACS) FILE COVERS 1967 - 19 Jun 1996 (960619/ED) VOL 124 ISS 26
Search both the L# from the Registry file (for the Registry number) and the name acetylcholine
=> s l1 or acetylcholine
21481 L1
40657 ACETYLCHOLINE
L2 41310 L1 OR ACETYLCHOLINE
Search the other two terms individually for maximum flexibility in combining them.
=> s receptor# L3 319187 RECEPTOR# => s antibod? L4 206193 ANTIBOD?
Combine the terms with the (s) “same sentence” operator for greater specificity than with AND
=> s L2 (s) L3 (s) L4 L5 757 L2 (S) L3 (S) L4
Display answer 3 of L5 in ibib format to give hints for other additional search terms.
=> d L5 ibib 3
L5 ANSWER 3 OF 757 CA COPYRIGHT 1996 ACS
ACCESSION NUMBER: 124:286753 CA
TITLE: A synthetic peptide corresponding to the second
extracellular loop of the human M2 acetylcholine
receptor induces pharmacological and morphological
changes in cardiomyocytesby active immunization
after 6 months in rabbits
AUTHOR(S): Fu, Michael L. X.; Schulze, Wolfgang; Wallukat,
Gerd; Hjalmarson, Aake; Hoebeke, Johan
CORPORATE SOURCE: Wallenberg Laboratory, Sahlgren’s
Hospital, Goeteborg, S-413 45, Swed.
SOURCE: Clin. Immunol. Immunopathol. (1996), 78(2),
203-7 CODEN: CLIIAT;
ISSN: 0090-1229
DOCUMENT TYPE: Journal
LANGUAGE: English
Search the additional terms found for acetylcholine-related receptors
=> s muscarinic or nicotinic or cholinergic
14983 MUSCARINIC
14753 NICOTINIC
29900 CHOLINERGIC
L6 49216 MUSCARINIC OR NICOTINIC OR CHOLINERGIC
Combine this set of answers with the set for “acetycholine” using OR, then combine with “receptor#” and “antibod?”
=> s (L2 or L6) (s) L3 (s) L4 L7 938 (L2 OR L6) (S) L3 (S) L4
Use Not to identify the new records not found in the search. Note that 181 more references were found with the added search terms.
=> s L7 not L5 L8 181 L7 NOT L5
Displays answer 1 of L8 in indented bibliographic format
=> d L8 ibib 1
L8 ANSWER 1 OF 181 CA COPYRIGHT 1996 ACS
ACCESSION NUMBER: 124:340916 CA
TITLE: Target specific screens and their use for
discovering small organic molecular
pharmacophores
INVENTOR(S): Blume, Arthur J.
PATENT ASSIGNEE(S): Dgi Technologies, Inc., USA
SOURCE: PCT Int. Appl., 202 pp.
CODEN: PIXXD2
NUMBER DATE
-------------- ------
PATENT INFORMATION: WO 9604557 A2 960215
DESIGNATED STATES: W: AM, AT, AU, BB, BG, BR,
BY, CA, CH, CN, CZ, DE, DK, EE, ES, FI, GB, GE, HU, IS,
JP, KE, KG, KP, KR, KZ, LK, LR, LT, LU, LV, MD, MG, MN,
MW, MX, NO, NZ, PL, PT, RO, RU, SD, SE, SG, SI, SK, TJ,
TM, TT RW: AT, BE, BF, BJ, CF, CG, CH, CI, CM, DE, DK,
ES, FR, GA, GB, GR, IE, IT, LU, MC, ML, MR, NE, NL, PT,
SE, SN, TD, TG
APPLICATION INFORMATION: WO 95-US10182 950802
PRIORITY APPLN. INFO.: US 94-286084 940803
US 95-473105 950607
DOCUMENT TYPE: Patent
LANGUAGE: English
Log off in one step
=> log y