@article {445,
	title = {β-catenin-mediated Wnt signal transduction proceeds through an endocytosis-independent mechanism.},
	journal = {Molecular biology of the cell},
	volume = {31},
	year = {2020},
	month = {2020 Jun 15},
	pages = {1425-1436},
	abstract = {The Wnt pathway is a key intercellular signaling cascade that regulates development, tissue homeostasis, and regeneration. However, gaps remain in our understanding of the molecular events that take place between ligand-receptor binding and target gene transcription. We used a novel tool for quantitative, real-time assessment of endogenous pathway activation, measured in single cells, to answer an unresolved question in the field-whether receptor endocytosis is required for Wnt signal transduction. We combined knockdown or knockout of essential components of clathrin-mediated endocytosis with quantitative assessment of Wnt signal transduction in mouse embryonic stem cells (mESCs). Disruption of clathrin-mediated endocytosis did not affect accumulation and nuclear translocation of β-catenin, as measured by single-cell live imaging of endogenous β-catenin, and subsequent target gene transcription. Disruption of another receptor endocytosis pathway, caveolin-mediated endocytosis, did not affect Wnt pathway activation in mESCs. Additional results in multiple cell lines support that endocytosis is not a requirement for Wnt signal transduction. We show that off-target effects of a drug used to inhibit endocytosis may be one source of the discrepancy among reports on the role of endocytosis in Wnt signaling.},
	issn = {1059-1524},
	doi = {10.1091/mbc.E20-02-0114},
	url = {https://www.molbiolcell.org/doi/10.1091/mbc.E20-02-0114?url_ver=Z39.88-2003\&rfr_id=ori:rid:crossref.org\&rfr_dat=cr_pub\%3dpubmed},
	author = {Rim, EY and Kinney, LK and Nusse, R}
}
