@article {cite-key,
	title = {Construction of a retroviral cDNA version of the int-1 mammary oncogene and its expression in vitro.},
	journal = {Nucleic Acids Res},
	volume = {14},
	number = {2},
	year = {1986},
	month = {Jan},
	pages = {693{\textendash}702},
	abstract = {The int-1 mammary oncogene is activated by proviruses of the Mouse Mammary Tumor Virus in many different mammary tumors. We have inserted a genomic fragment containing the protein-encoding domain of the gene into the retroviral shuttle vector pZIPneoSV(X)1. After one round of virus replication we recovered recombinant proviral DNA containing a correctly spliced copy of int-1. In vitro transcription of this cDNA version of int-1 using SP6 polymerase and translation in a reticulocyte lysate yielded a protein of approximately 37,000 daltons. High expression of int-1 in NIH-3T3 cells infected with recombinant virus did not lead to morphological transformation.},
	issn = {0305-1048 (Print); 0305-1048 (Linking)},
	author = {Rijsewijk, F A and van Lohuizen, M and van Ooyen, A and Nusse, R}
}
