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William Noderer
Chemical Engineering
Bio-X
Graduate Student Fellowships 2010/2011
Professor Cliff Wang (Chemical Engineering)
The transcription factor p53 is a potent tumor suppressor. γ-irradiation-induced DNA damage can cause oscillations of p53. Does oscillatory behavior aid the cells in responding to DNA damage? William will create an inducible system to manually control p53 oscillations. p53 will be fused to an inducible destabilizing domain and my constructs will be introduced into p53-/- Mdm2-/- mouse embryonic fibroblasts (MEF).
Using a microfluidic device to control the concentration of a small molecule inducer, William will generate oscillations in p53. The experiment will directly show how p53 oscillations, when compared with constant levels, affect cell survival and gene expression. |
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