This alignment (RN, 7/15/03) is based on most available Mouse and Drosophila Wnt proteins.

MOUSEWNT1                    ------------------MGLWALLPSWVSTTLLLALTALPAALAANSSG
MOUSEWNT2                    --------------------------------MNVPLGGIWLWLPLLLTW
MOUSEWNT2B                   MLKLQGEDEAAQLAPRRARVPVPRPTAPDVSPSSARLGLACLLLLLLLT-
MOUSEWNT3                    ------------------------MEPHLLGLLLGLLLSGTRVLAGYPIW
MOUSEWNT3A                   ------------------------MAP--LGYLL-VLCSLKQALGSYPIW
MOUSEWNT4                    ------------------------------MSPRSCLRSLRLLVFAVFSA
MOUSEWNT5A                   ----MKKPIGILSPGVALGTAGGAMSSKFFLMALATFFSFAQVVIEANSW
MOUSEWNT5B                   ----------MLVPG-HWDGLRPAMPSLLLVVVAALLSSWAQLLTDANSW
MOUSEWNT6                    --------------------MLPPVPSRL-------GLLLLLLCPAHVDG
MOUSEWNT7A                   ------------------------------MTRKARRCLGHLFLSLGIVY
MOUSEWNT7B                   ------------------------------MHRNFRKWIFYVFLCFGVLY
MOUSEWNT8A                   -----------------------------------MGHLLMLWVAAGMCY
MOUSEWNT8B                   -------------------------------------MFLMKPVCVLLVT
MOUSEWNT9A                   ----------------------MLDGSLLARWLAAAFGLTLLLAALRPSA
MOUSEWNT9B                   --------------------------MRPAPALALAALCLLVLPAAAAAA
MOUSEWNT10A                  -----------MGSAHPRPWLRLPQGPQPRPEFWALLFFLLLLAAAVPRS
MOUSEWNT10B                  ----------------------MLEEPRSRPPPLGLAGLLFL--ALFSRA
MOUSEWNT11                   --------------------------MRARPQVCEALLFALALHTGVCYG
MOUSEWNT16                   ------------------------MDRAALLALPSLCALWAAVLSLLPCG
DrosophilaWg                 ------------------MDISYIFVICL-MALCSGGSSLSQVEGKQKSG
DrosophilaWnt2               --------------------------------------MWKIHNKLLIYI
DrosophilaWnt3/5_short_      --------------------------------------------------
hydraWnt                     ------------------------MGT--TRYKETLLCFLLIFMETQAQL
C_elegans_egl20              ----------------------MQFFICLIFLFVLLVYGQYSPPSATYST
			

MOUSEWNT1 RWWGIVNIASST-------NLLTDSKSL----QLVLEPSLQ-LLSRKQRR MOUSEWNT2 LTPEVSSSWWYM-------RATGGS--S----RVMCDNVPG--LVSRQRQ MOUSEWNT2B LPARVDTSWWYI-------GALG----A----RVICDNIPG--LVSRQRQ MOUSEWNT3 WSLALGQQYTSL-------ASQPL----------LCGSIPG--LVPKQLR MOUSEWNT3A WSLAVGPQYSSL-------STQPI----------LCASIPG--LVPKQLR MOUSEWNT4 AASNWLYLAKLS-------SVGSIS--E----EETCEKLKG--LIQRQVQ MOUSEWNT5A WSLGMNNPVQMS-------EVYIIG--A----QPLCSQLAG--LSQGQKK MOUSEWNT5B WSLALN-PVQRP-------EMFIIG--A----QPVCSQLPG--LSPGQRK MOUSEWNT6 LWWA---------------VGSPLVMDP----TSICRKARR--LAGRQAE MOUSEWNT7A LRIGGFSSVVAL-------GASII-----------CNKIPG--LAPRQRA MOUSEWNT7B VKLGALSSVVAL-------GANII-----------CNKIPG--LAPRQRA MOUSEWNT8A PALGAS----------------------------------AWSVNNFLIT MOUSEWNT8B CVLHRSH---------------------------------AWSVNNFLMT MOUSEWNT9A AYFGLTGSEPLTILPL--TLETEAAAQA----HYKACDRLK--LERKQRR MOUSEWNT9B AYFGLTGREVLTPFPGLGTAAAPAQAGA----HLKQCDLLK--LSRRQKQ MOUSEWNT10A APNDILGLRLP---------PEPVLNAN----TVCL-TLPG--LSRRQME MOUSEWNT10B LSNEILGLKLP---------GEPPLTAN----TVCL-TLSG--LSKRQLG MOUSEWNT11 IKWLALSKTPA--------ALALNQTQH----CKQ---LEG--LVSAQVQ MOUSEWNT16 TQGNWMWLGIASFGVPEKLGCADLP------------------LNSRQKE DrosophilaWg RGRGSMWWGIAK-------VGEPNNITP----IMYMDPAIHSTLRRKQRR DrosophilaWnt2 LWIMEIRLVSSF-------TSAML-----------CGRIPG--LTPGQRN DrosophilaWnt3/5_short_ ----------------------------------MCYSAIG--LSNSQKK hydraWnt W-MALGTQTSAI-------ESRPRSSIN----KNLCRALY---LHHYQRT C_elegans_egl20 QQVPYNWLTLAFVGSNDYLPRPHYHSTDREHFKELCRRLDG--LNPNQQA

MOUSEWNT1 LIRQNPGILHSVSGGLQSAVRECKWQFRNRRWNCPT---------APGPH MOUSEWNT2 LCHRHPDVMRAIGLGVAEWTAECQHQFRQHRWNCNTL------DR--DHS MOUSEWNT2B LCQRYPDIMRSVGEGAREWIRECQHQFRHHRWNCTTL------DR--DHT MOUSEWNT3 FCRNYIEIMPSVAEGVKLGIQECQHQFRGRRWNCTTI------DD--SLA MOUSEWNT3A FCRNYVEIMPSVAEGVKAGIQECQHQFRGRRWNCTTV------SN--SLA MOUSEWNT4 MCKRNLEVMDSVRRGAQLAIEECQYQFRNRRWNCSTL------D---SLP MOUSEWNT5A LCHLYQDHMQYIGEGAKTGIKECQYQFRHRRWNCSTV------D---NTS MOUSEWNT5B LCQLYQEHMSYIGEGAKTGIRECQHQFRQRRWNCSTV------D---NTS MOUSEWNT6 LCQAEPEVVAELARGARLGVRECQFQFRFRRWNCSS-----------HSK MOUSEWNT7A ICQSRPDAIIVIGEGSQMGLDECQFQFRNGRWNCSAL------GE--R-T MOUSEWNT7B ICQSRPDAIIVIGEGAQMGIDECQHQFRFGRWNCSAL------GE--K-T MOUSEWNT8A GPKAYLTYTASVALGAQIGIEECKFQFAWERWNCPEH---------AFQF MOUSEWNT8B GPKAYLVYSSSVAAGAQSGIEECKYQFAWDRWNCPER---------ALQL MOUSEWNT9A MCRRDPGVAETLVEAVSMSALECQYQFRFERWNCTLE------GR----- MOUSEWNT9B LCRREPGLAETLRDAAHLGLLECQFQFRQERWNCSLE------G------ MOUSEWNT10A VCVRHPDVAASAIQGIQIAIHECQHQFRDQRWNCSSL------ETRNKVP MOUSEWNT10B LCLRSPDVTASALQGLHIAVHECQHQLRDQRWNCSAL------EGGGRLP MOUSEWNT11 LCRSNLELMRTIVHAARGAMKACRRAFADMRWNCSSI------ELA---P MOUSEWNT16 LCKRKPYLLPSIREGARLGIQECRSQFRHERWNCMV--ATTTSTQLATAP DrosophilaWg LVRDNPGVLGALVKGANLAISECQHQFRNRRWNCSTR------NFSRGKN DrosophilaWnt2 MCREMPDALIALGEGHQLGAQECQHQFRGHRWNCSEV------WQ--R-N DrosophilaWnt3/5_short_ QCVKHTSVMPAISRGARAAIQECQFQFKNRRWNCSTT------N---DET hydraWnt VCLNYTDLMLSVAEGIRLGIDECQVQFKHRKWNCTIN------EH--GTS C_elegans_egl20 LCAENPFSIPFVARGVREAIRECENKFKFERWNCSSRDEVTETRHGKFQD

MOUSEWNT1 LFGKIVNRGCRETAFIFAITSAGVTHSVARSCSEGSIESCTCDYRRRG-- MOUSEWNT2 LFGRVLLRSSRESAFVYAISSAGVVFAITRACSQGELKSCSCDPKKKG-- MOUSEWNT2B VFGRAMLRSSREAAFVYAISSAGVVHAITRACSQGELSVCSCDPYTRG-- MOUSEWNT3 IFGPVLDKATRESAFVHAIASAGVAFAVTRSCAEGTSTICGCDSHHK--- MOUSEWNT3A IFGPVLDKATRESAFVHAIASAGVAFAVTRSCAEGSAAICGCSSRLQ--- MOUSEWNT4 VFGKVVTQGTREAAFVYAISSAGVAFAVTRACSSGELEKCGCDRTV---- MOUSEWNT5A VFGRVMQIGSRETAFTYAVSAAGVVNAMSRACREGELSTCGCSRA----- MOUSEWNT5B VFGRVMQIGSRETAFTYAVSAAGVVNAISRACREGELSTCGCSRAA---- MOUSEWNT6 AFGRVLQQDIRETAFVFAITAAGASHAVTQACSMGELLQCGCQAPRGRAP MOUSEWNT7A VFGKELKVGSREAAFTYAIIAAGVAHAITAACTQGNLSDCGCDKEKQ--- MOUSEWNT7B VFGQELRVGSREAAFTYAITAAGVAHAVTAACSQGNLSNCGCDREKQ--- MOUSEWNT8A STHNRLRAATRETSFIHAIRSAAIMYAVTKNCSMGDLENCGCDESQ---- MOUSEWNT8B SSHGGLRSANRETAFVHAISSAGVMYTLTRNCSLGDFDNCGCDDSR---- MOUSEWNT9A YRASLLKRGFKETAFLYAISSAGLTHALAKACSAGRMERCTCD------- MOUSEWNT9B -RTGLLQRGFKETAFLYAVSAAALTHALARACSAGRMERCTCD------- MOUSEWNT10A YESPIFSRGFRESAFAYAIAAAGVVHAVSNACALGKLKACGCDASRRGDE MOUSEWNT10B HHSAILKRGFRESAFSFSMLAAGVMHAVATACSLGKLVSCGCGWKGSGEQ MOUSEWNT11 NYLLDLERGTRESAFVYALSAATISHTIARACTSGDLPGCSCG------- MOUSEWNT16 LFGYELSSGTKETAFIYAIMAAGLVHSVTRSCSAGNMTECSCDTT----- DrosophilaWg LFGKIVDRGCRETSFIYAITSAAVTHSIARACSEGTIESCTCDYSHQSRS DrosophilaWnt2 VFAHVIPTASREAAYTYAIASAGAAYRVTAACARGNISTCGCDVRHKATP DrosophilaWnt3/5_short_ VFGPMTSLAAPEMAFIHALAAATVTSFIARACRDGQLASCSCSRGS---- hydraWnt VFGPIITTASRESAFISGIISAGVAFSVTESCAEGKSVHCRCDNSVR--- C_elegans_egl20 ILGKTLRSANKEAAFLNAIMAASIVHSITKGCNTGNLTECGCDSKPGMQR

MOUSEWNT1 ------------------------------------PG--GPDWHWGGCS MOUSEWNT2 ---------------------------------SAKDS--KGTFDWGGCS MOUSEWNT2B ---------------------------------RHHDQ--RGDFDWGGCS MOUSEWNT3 -----------------------------------GPP--GEGWKWGGCS MOUSEWNT3A -----------------------------------GSP--GEGWKWGGCS MOUSEWNT4 ---------------------------------HGVS---PQGFQWSGCS MOUSEWNT5A ---------------------------------RPKDL--PRDWLWGGCG MOUSEWNT5B ---------------------------------RPKDL--PRDWLWGGCG MOUSEWNT6 PRPSGLL-----------------GTPGPPGPTGSPDA--SAAWEWGGCG MOUSEWNT7A ---------------------------------GQYHW--DEGWKWGGCS MOUSEWNT7B ---------------------------------GYYNQ--AEGWKWGGCS MOUSEWNT8A -----------------------------------NGKTGGHGWIWGGCS MOUSEWNT8B -----------------------------------NGQLGGQGWLWGGCS MOUSEWNT9A -------------------------------EAPDLEN--REAWQWGGCG MOUSEWNT9B -------------------------------DSPGLES--RQAWQWGVCG MOUSEWNT10A EAFRRKLHRLQLDALQRGKGLSHGVPEHPAILPASPGL--QDSWEWGGCS MOUSEWNT10B DRLRAKL--LQLQALSRGKTFPISQPSPVPGSVPSPGP--QDTWEWGGCN MOUSEWNT11 -------------------------------PVPGEPP--GPGNRWGGCA MOUSEWNT16 ------------------------------LQNGGSPS---EGWHWGGCS DrosophilaWg PQANH--------------------------QAGSVAG--VRDWEWGGCS DrosophilaWnt2 --------------------------------TGGGTP--DEPWKWGGCS DrosophilaWnt3/5_short_ ---------------------------------RPKQL--HDDWKWGGCG hydraWnt -----------------------------------GQT--DEGWRWGGCN C_elegans_egl20 ------------------------------YQAESDPSMSRDQFSWGGCS

MOUSEWNT1 DNIDFGRLFGREFVDSGEKGR--------DLRFLMNLHNNEAGRTTVFSE MOUSEWNT2 DNIDYGIKFARAFVDAKERK-------GKDARALMNLHNNRAGRKAVKRF MOUSEWNT2B DNIHYGVRFAKAFVDAKEKR-------LKDARALMNLHNNRCGRTAVRRF MOUSEWNT3 EDADFGVLVSREFADAREN--------RPDARSAMNKHNNEAGRTTILDH MOUSEWNT3A EDIEFGGMVSREFADAREN--------RPDARSAMNRHNNEAGRQAIASH MOUSEWNT4 DNIAYGVAFSQSFVDVRERSK-----GASSSRALMNLHNNEAGRKAILTH MOUSEWNT5A DNIDYGHPFAKEFVDARERERIHAKGSYESARILMNLHNNEAGRRTVYNL MOUSEWNT5B DNVEYGYRFAKEFVDAREREKNFAKGSEEQGRALMNLQNNEAGRRAVYKM MOUSEWNT6 DDVDFGDEKSRLFMDAQHKRGR-----G-DIRALVQLHNNEAGRLAVRSH MOUSEWNT7A ADIRYGIGFAKVFVDAREI--------KQNARTLMNLHNNEAGRKILEEN MOUSEWNT7B ADVRYGIDFSRRFVDAREI--------KKNARRLMNLHNNEAGRKVLEDR MOUSEWNT8A DNVEFGEKISRLFVDSLEK--------GKDARALVNLHNNRAGRLAVRAS MOUSEWNT8B DNVGFGEAISKQFVDALET--------GQDARAAMNLHNNEAGRKAVKGT MOUSEWNT9A DNLKYSSKFVKEFLG---------RRSSKDLRARVDFHNNLVGVKVIKAG MOUSEWNT9B DNLKYSTKFLSNFLGP--------KRGSKDLRARADAHNTHVGIKAVKSG MOUSEWNT10A PDVGFGERFSKDFLDS--------REPHRDIHARMRLHNNRVGRQAVMEN MOUSEWNT10B HDMDFGEKFSRDFLDS--------REAPRDIQARMRIHNNRVGRQVVTEN MOUSEWNT11 DNLSYGLLMGAKFSDAPMKV----KKTGSQANKLMRLHNSEVGRQALRAS MOUSEWNT16 DDVQYGMWFSRKFLDLPIRNT---TGKESRVLLAMNLHNNEAGRQAVAKL DrosophilaWg DNIGFGFKFSREFVDTGERGR--------NLREKMNLHNNEAGRAHVQAE DrosophilaWnt2 ADVDFGMRYARRFMDAREL--------ERDSRTLMNLHNNRAGRTLVKKM DrosophilaWnt3/5_short_ DNLEFAYKFATDFIDSREKE------TNVKARSLMNLHNNEAGRRAVIKK hydraWnt RPITYGIWFSQLFIDQVEKIVK----KRKDPRKIMNLHNNKAGREVIKNL C_elegans_egl20 DNVPHGIRYAKKFLDDWETAQ---FDKTKNVAHLVRRHNNFVGREAIAQN

MOUSEWNT1 MRQECKCHGMSGSCTVRTCWMRLPTLRAVGDVLRDRFDGASRVLYGNRGS MOUSEWNT2 LKQECKCHGVSGSCTLRTCWLAMADFRKTGEYLWRKYNGAIQVVMNQDGT MOUSEWNT2B LKLECKCHGVSGSCTLRTCWRALSDFRRTGDYLRRRYDGAVQVTATQDGA MOUSEWNT3 MHLKCKCHGLSGSCEVKTCWWAQPDFRAIGDFLKDKYDSASEMVVEKHRE MOUSEWNT3A MHLKCKCHGLSGSCEVKTCWWSQPDFRTIGDFLKDKYDSASEMVVEKHRE MOUSEWNT4 MRVECKCHGVSGSCEVKTCWRAVPPFRQVGHALKEKFDGATEVEPRRVGS MOUSEWNT5A ADVACKCHGVSGSCSLKTCWLQLADFRKVGDALKEKYDSAAAMRLNSRGK MOUSEWNT5B ADVACKCHGVSGSCSLKTCWLQLAEFRKVGDRLKEKYDSAAAMRITRQGK MOUSEWNT6 TRTECKCHGLSGSCALSTCWQKLPPFREVGARLLERFHGASRV------- MOUSEWNT7A MKLECKCHGVSGSCTTKTCWTTLPQFRELGYVLKDKYNEAVHVEPVRASR MOUSEWNT7B MKLECKCHGVSGSCTTKTCWTTLPKFREVGHLLKEKYNAAVQVEVVRASR MOUSEWNT8A TKRTCKCHGISGSCSIQTCWLQLADFRQMGNYLKAKYDRALKIEMDKRQL MOUSEWNT8B MKRTCKCHGVSGSCTTQTCWLQLPEFREVGAHLKEKYHAALKVDL--LQ- MOUSEWNT9A VETTCKCHGVSGSCTVRTCWRQLAPFHEVGKHLKHKYETSLKVGSTTNEA MOUSEWNT9B LRTTCKCHGVSGSCAVRTCWKQLSPFRETGQVLKLRYDTAVKVSSATNEA MOUSEWNT10A MRRKCKCHGTSGSCQLKTCWQVTPEFRTVGALLRNRFHRATLIRPHNRNG MOUSEWNT10B LKRKCKCHGTSGSCQFKTCWRAAPEFRAIGAALRERLSRAIFIDTHNRNS MOUSEWNT11 LETKCKCHGVSGSCSIRTCWKGLQELQDVAADLKTRYLSATKVVHRPMGT MOUSEWNT16 MSVDCRCHGVSGSCAVKTCWKTMSSFEKIGHFLKDKYEN-SIQISDKTKR DrosophilaWg MRQECKCHGMSGSCTVKTCWMRLANFRVIGDNLKARFDGATRV----QVT DrosophilaWnt2 LRTDCKCHGVSGSCVMKTCWKSLPPFRLVGDRLMMKYQKAKTVQAVKGKR DrosophilaWnt3/5_short_ ARITCKCHGVSGSCSLITCWQQLSSIREIGDYLREKYEGATKVKINKRGR hydraWnt LQTECKCHGTSGNCNLKTCWRSQPHFSEIGKILKEKYDSAHEMEFLYKVK C_elegans_egl20 IRRQCRCHGVSGSCEFKTCWLQMQKFSQVSDLLKKRYDHFAVQVTRKATK

MOUSEWNT1 NRASRAELLRLEPEDPAHKP-----PSPHDLVYFEKSPNFCTYSGRLGTA MOUSEWNT2 G---------FTVANKRFKK-----PTKNDLVYFENSPDYCIRDREAGSL MOUSEWNT2B N---------FTAARQGYRH-----ATRTDLVYFDNSPDYCVLDKASGSL MOUSEWNT3 S-RG--WVETLRAKYALFKP-----PTERDLVYYENSPNFCEPNPETGSF MOUSEWNT3A S-RG--WVETLRPRYTYFKV-----PTERDLVYYEASPNFCEPNPETGSF MOUSEWNT4 S-RA------LVPRNAQFKP-----HTDEDLVYLEPSPDFCEQDIRSGVL MOUSEWNT5A ----------LVQVNSRFNS-----PTTQDLVYIDPSPDYCVRNESTGSL MOUSEWNT5B ----------LELANSRFNQ-----PTPEDLVYVDPSPDYCLRNETTGSL MOUSEWNT6 --MGTNDGKALLPAVRTLKP-----PGRADLLYAADSPDFCAPNRRTGSP MOUSEWNT7A N-KRPTFLKIKKPLSYRK-------PMDTDLVYIELSPNYCEEDPVTGSV MOUSEWNT7B L-RQPTFLRIKQLRSYQK-------PMETDLVYIEKSPNYCEEDAATGSV MOUSEWNT8A --RAGNRAEGRWALTEAFLP-----STEAELIFLEGSPDYCNRNASLSIQ MOUSEWNT8B --GAGNSAAGRGAIADTFRS-----ISTRELVHLEDSPDYCLENKTLGLL MOUSEWNT9A TGEAGAISPPRGRASGSGGGD--PLPRTPELVHLDDSPSFCLAGRF--SP MOUSEWNT9B LGRLELWAPAKPGGPAKG-----LAPRPGDLVYMEDSPSFCRPSKY--SP MOUSEWNT10A GQLEPGPAGAPSPAPGTPGLR--RRASHSDLVYFEKSPDFCEREPRLDSA MOUSEWNT10B GAFQP-------------RLR--PRRLSGELVYFEKSPDFCERDPTLGSP MOUSEWNT11 RKHLVPKDLDIRPVKDSE------------LVYLQSSPDFCMKNEKVGSH MOUSEWNT16 NMRRREKDQRQTPI------------LKDDLLYVHKSPNYCVENKKLGIP DrosophilaWg NSLRATYHFQLNPHNPEHKP-----PGSKDLVYLEPSPSFCEKNLRQGIL DrosophilaWnt2 G-LRLVLSRKKHAGTARAQKPVLDWPKRMELIYLEASPNYCERSLQTGSQ DrosophilaWnt3/5_short_ ----------LQIKDLQFKV-----PTAHDLIYLDESPDWCRNSYALHWP hydraWnt A-NGERKIKDLIPKYKEYLP-----PSSLDFIYYEESPNYVK-NETLGIA C_elegans_egl20 RLRRKERTERKIPL------------RGNEMAYVHRSPSYCEKNLTAGIL

MOUSEWNT1 GTAGRACNSSSPALD-----GCELLC--CGRGHRTRTQRVTERCNCTFHW MOUSEWNT2 GTAGRVCNLTSRGMD-----SCEVMC--CGRGYDTSHVTRMTKCECKFHW MOUSEWNT2B GTAGRVCSKTSKGTD-----GCEIMC--CGRGYDTTRVTRVTQCECKFHW MOUSEWNT3 GTRDRTCNVTSHGID-----GCDLLC--CGRGHNTRTEKRKEKCHCVFHW MOUSEWNT3A GTRDRTCNVSSHGID-----GCDLLC--CGRGHNARTERRREKCHCVFHW MOUSEWNT4 GTRGRTCNKTSKAID-----GCELLC--CGRGFHTAQVELAERCGCRFHW MOUSEWNT5A GTQGRLCNKTSEGMD-----GCELMC--CGRGYDQFKTVQTERCHCKFHW MOUSEWNT5B GTQGRLCNKTSEGMD-----GCELMC--CGRGYDRFKSVQVERCHCRFHW MOUSEWNT6 GTRGRACNSSAPDLS-----GCDLLC--CGRGHRQESVQLEENCLCRFHW MOUSEWNT7A GTQGRACNKTAPQAS-----GCDLMC--CGRGYNTHQYARVWQCNCKFHW MOUSEWNT7B GTQGRLCNRTSPGAD-----GCDTMC--CGRGYNTHQYTKVWQCNCKFHW MOUSEWNT8A GTEGRECLQNARSASRREQRSCGRLCTECGLQVEERRAEAVSSCDCNFQW MOUSEWNT8B GTEGRECLRRGRALGRWERRSCRRLCGDCGLAVEERRAETVSSCNCKFHW MOUSEWNT9A GTAGRRCHREKN---------CESIC--CGRGHNTQSRVVTRPCQCQVRW MOUSEWNT9B GTAGRVCSRDSS---------CSSLC--CGRGYDTQSRMVVFSCHCQVQW MOUSEWNT10A GTVGRLCNKSSTGPD-----GCGSMC--CGRGHNILRQTRSERCHCRFHW MOUSEWNT10B GTRGRACNKTSRLLD-----GCGSLC--CGRGHNVLRQTRVERCHCRFHW MOUSEWNT11 GTQDRQCNKTSNGSD-----SCDLMC--CGRGYNPYTDRVVERCHCKYHW MOUSEWNT16 GTQGRECNRTSGGAD-----GCNLLC--CGRGYNTHVVRHVERCECKFIW DrosophilaWg GTHGRQCNETSLGVD-----GCGLMC--CGRGYRRDEVVVVERCACTFHW DrosophilaWnt2 GTSGRTCQRTGHGPQ-----SCDLLC--CGRGHNTQHIRRTTQCRCQFRW DrosophilaWnt3/5_short_ GTHGRVCHKNSSGLE-----SCAILC--CGRGYNTKNIIVNERCNCKFHW hydraWnt GTKGRSCNITSSGVD-----GCELMC--CQRGYNVNIVQKTHSCECKFVW C_elegans_egl20 GTSGRECIHNSYSSE-----SCDLLC--CGRGYNTRLEIRQTQCECKFVW

MOUSEWNT1 CCHVSCRNCTHTRVLHECL----------------- MOUSEWNT2 CCAVRCQDCLEALDVHTCKAPKSADWATPT------ MOUSEWNT2B CCAVRCKECRNTVDVHTCKAPKKAEWLDQT------ MOUSEWNT3 CCYVSCQECIRIYDVHTCK----------------- MOUSEWNT3A CCYVSCQECTRVYDVHTCK----------------- MOUSEWNT4 CCFVKCRQCQRLVEMHTCR----------------- MOUSEWNT5A CCYVKCKKCTEIVDQFVCK----------------- MOUSEWNT5B CCFVRCKKCTEVVDQYVCK----------------- MOUSEWNT6 CCVVQCHRCRVRKELSLCL----------------- MOUSEWNT7A CCYVKCNTCSERTEMYTCK----------------- MOUSEWNT7B CCFVKCNTCSERTEVFTCK----------------- MOUSEWNT8A CCTVKCGQCRRVVSRYYCTRPVGSARPRGRGKDSAW MOUSEWNT8B CCAVRCEQCRRRVTKYFCSRAERPPRGAAHKPGKNS MOUSEWNT9A CCYVECRQCTQREEVYTCKG---------------- MOUSEWNT9B CCYVECQQCAQQELVYTCKR---------------- MOUSEWNT10A CCFVVCEECRITEWVSVCK----------------- MOUSEWNT10B CCYVLCDECKVTEWVNVCK----------------- MOUSEWNT11 CCYVTCRRCERTVERYVCK----------------- MOUSEWNT16 CCYVRCRRCESMTDVHTCK----------------- DrosophilaWg CCEVKCKLCRTKKVIYTCL----------------- DrosophilaWnt2 CCEVKCDECDESYEEFTCK----------------- DrosophilaWnt3/5_short_ CCQVKCEVCTKVLEEHTCK----------------- hydraWnt CCKVSCNSCIKMTPEYTCK----------------- C_elegans_egl20 CCEVKCKTCTEEVAVHTCK-----------------